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KMID : 0881720100250040388
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Journal of Food Hygiene and Safety
2010 Volume.25 No. 4 p.388 ~ p.394
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Method Development for the Profiling Analysis of Endogenous Metabolites by Accurate-Mass Quadrupole Time-of-Flight(Q-TOF) LC/MS
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Lee In-Sun
Kim Jin-Ho
Cho Soo-Yeul
Shim Sun-Bo
Park Hye-Jin
Lee Jin-Hee
Lee Ji-Hyun
Hwang In-Sun
Kim Sung-Il
Lee Jung-Hee
Cho Su-Yeon
Choi Don-Woong
Cho Yang-Ha
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Abstract
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Metabolomics aims at the comprehensive, qualitative and quantitative analysis of wide arrays of endogenous metabolites in biological samples. It has shown particular promise in the area of toxicology and drug development, functional genomics, system biology and clinical diagnosis. In this study, analytical technique of MS instrument with high resolution mass measurement, such as time-of-flight (TOF) was validated for the purpose of investigation of amino acids, sugars and fatty acids. Rat urine and serum samples were extracted by selected each solvent (50% acetonitrile, 100% acetonitrile, acetone, methanol, water, ether) extraction method. We determined the optimized liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) system and selected appropriated columns, mobile phases, fragment energy and collision energy, which could search 17 metabolites. The spectral data collected from LC/TOFMS were tested by ANOVA. Obtained with the use of LC/TOFMS technique, our results indicated that (1) MS and MS/MS parameters were optimized and most abundant product ion of each metabolite were selected to be monitorized; (2) with design of experiment analysis, methanol yielded the optimal extraction efficiency. Therefore, the results of this study are expected to be useful in the endogenous metabolite fields according to validated SOP for endogenous amino acids, sugars and fatty acids.
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KEYWORD
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Metabolomics, LC/TOFMS, solvent extraction
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